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blunt end cloning vector  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co blunt end cloning vector
    Blunt End Cloning Vector, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ta+blunt+zero+cloning+kit/5+min+TA+Blunt-Zero+Cloning+Kit/pmc12925555-64-13-16
    Average 96 stars, based on 290 article reviews
    blunt end cloning vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Development of canine parvovirus-neutralizing monoclonal antibodies from natural host and their germline gene usage.
    Article Snippet: .. The PCR products were cloned into the pCE2 TA/Blunt-Zero cloning vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Cat# C601), following the manufacturer’s instructions. ..

    Article Title: Development of canine parvovirus-neutralizing monoclonal antibodies from natural host and their germline gene usage
    Article Snippet: .. The PCR products were cloned into the pCE2 TA/Blunt-Zero cloning vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Cat# C601), following the manufacturer’s instructions. ..

    Article Title: Molecular basis of the short- and long-term osmoregulation capability in the euryhaline unicellular eukaryote Paramecium calkinsi
    Article Snippet: .. The PCR products were subsequently ligated into the pCE2 TA/Blunt-Zero vector using a 5 min TA/Blunt-Zero Cloning Kit (Vazyme, China, Cat. No. C601-02), and then transferred to E. coli DH5α receptor cells. ..

    Article Title: Molecular basis of the short- and long-term osmoregulation capability in the euryhaline unicellular eukaryote Paramecium calkinsi
    Article Snippet: .. The open-reading frames of gene TPR, as well as putative regulatory regions, were amplified by PCR and cloned into the pCE2 TA/Blunt-Zero vector using 5 min TA/Blunt-Zero Cloning Kit (Vazyme, China, Cat. No. C601-02). .. The codon-optimized GFP (723 bp, accession number AB071703 in GenBank [ ]) was inserted after the start codon ATG by ligation of ends generated by the BsaI restriction enzyme (NEB, USA, Cat. No. R3733V).

    Article Title: SRBSDV-derived small interfering RNAs modulate insect vector reproduction and viral replication in Sogatella furcifera.
    Article Snippet: The primers used in this study were listed in Supplementary Table 1. dsRNA synthesis and injection Template fragments (approximately 500 bp) for dsRNA targeting SfTPS1, SfTPS2, SfTre1, SfTre2, SfTret1, SfTret1-2, SfGP, SfHK, SfToll8 and EGFP were synthesized using 2 × Phanta Max Master Mix (Vazyme Biotech, Nanjing, China), with primers listed in Supplementary Table 1. .. Following separation by agarose gel electrophoresis, PCR products of the expected size were excised, purified, and ligated into the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Nanjing, China). .. Single monoclonal plaques were selected and sent for sequencing (Tsingke Biotechnology, Beijing, China).

    Clone Assay:

    Article Title: Development of canine parvovirus-neutralizing monoclonal antibodies from natural host and their germline gene usage.
    Article Snippet: .. The PCR products were cloned into the pCE2 TA/Blunt-Zero cloning vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Cat# C601), following the manufacturer’s instructions. ..

    Article Title: Development of canine parvovirus-neutralizing monoclonal antibodies from natural host and their germline gene usage
    Article Snippet: .. The PCR products were cloned into the pCE2 TA/Blunt-Zero cloning vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Cat# C601), following the manufacturer’s instructions. ..

    Article Title: Molecular basis of the short- and long-term osmoregulation capability in the euryhaline unicellular eukaryote Paramecium calkinsi
    Article Snippet: .. The open-reading frames of gene TPR, as well as putative regulatory regions, were amplified by PCR and cloned into the pCE2 TA/Blunt-Zero vector using 5 min TA/Blunt-Zero Cloning Kit (Vazyme, China, Cat. No. C601-02). .. The codon-optimized GFP (723 bp, accession number AB071703 in GenBank [ ]) was inserted after the start codon ATG by ligation of ends generated by the BsaI restriction enzyme (NEB, USA, Cat. No. R3733V).

    Cloning:

    Article Title: Development of canine parvovirus-neutralizing monoclonal antibodies from natural host and their germline gene usage.
    Article Snippet: .. The PCR products were cloned into the pCE2 TA/Blunt-Zero cloning vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Cat# C601), following the manufacturer’s instructions. ..

    Article Title: Development of canine parvovirus-neutralizing monoclonal antibodies from natural host and their germline gene usage
    Article Snippet: .. The PCR products were cloned into the pCE2 TA/Blunt-Zero cloning vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Cat# C601), following the manufacturer’s instructions. ..

    Article Title: Prolactin-PRLR-STAT5 signaling upregulates a candidate pheromone receptor caor67 in the olfactory epithelium of northern snakehead (Channa argus).
    Article Snippet: Pheromones are key modulators of reproductive behavior in fish, and certain odorant receptors (ORs) expressed in the olfactory epithelium act as receptors for sex pheromones.. The northern snakehead (Channa argus) exhibits distinctive parental care behaviors, such as nest construction and brood guarding.. It is likely that hormonal regulation during the breeding season upregulates the expression of olfactory receptors, thereby enhancing the sensitivity of C. argus to pheromones released by the opposite sex and ultimately promoting the initiation of reproductive behaviors.

    Article Title: Molecular basis of the short- and long-term osmoregulation capability in the euryhaline unicellular eukaryote Paramecium calkinsi
    Article Snippet: .. The PCR products were subsequently ligated into the pCE2 TA/Blunt-Zero vector using a 5 min TA/Blunt-Zero Cloning Kit (Vazyme, China, Cat. No. C601-02), and then transferred to E. coli DH5α receptor cells. ..

    Article Title: Molecular basis of the short- and long-term osmoregulation capability in the euryhaline unicellular eukaryote Paramecium calkinsi
    Article Snippet: .. The open-reading frames of gene TPR, as well as putative regulatory regions, were amplified by PCR and cloned into the pCE2 TA/Blunt-Zero vector using 5 min TA/Blunt-Zero Cloning Kit (Vazyme, China, Cat. No. C601-02). .. The codon-optimized GFP (723 bp, accession number AB071703 in GenBank [ ]) was inserted after the start codon ATG by ligation of ends generated by the BsaI restriction enzyme (NEB, USA, Cat. No. R3733V).

    Article Title: SRBSDV-derived small interfering RNAs modulate insect vector reproduction and viral replication in Sogatella furcifera.
    Article Snippet: The primers used in this study were listed in Supplementary Table 1. dsRNA synthesis and injection Template fragments (approximately 500 bp) for dsRNA targeting SfTPS1, SfTPS2, SfTre1, SfTre2, SfTret1, SfTret1-2, SfGP, SfHK, SfToll8 and EGFP were synthesized using 2 × Phanta Max Master Mix (Vazyme Biotech, Nanjing, China), with primers listed in Supplementary Table 1. .. Following separation by agarose gel electrophoresis, PCR products of the expected size were excised, purified, and ligated into the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Nanjing, China). .. Single monoclonal plaques were selected and sent for sequencing (Tsingke Biotechnology, Beijing, China).

    Article Title: Functional 5Bq allele is responsible for the compact spike phenotype in common wheat ( Triticum aestivum L.)
    Article Snippet: .. Amplified target fragments were inserted into the pCE2 TA/Blunt-Zero vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme). .. Positive colonies were sequenced by Sangon Biotech (Chengdu, China).

    other:

    Article Title: Functional 5Bq allele is responsible for the compact spike phenotype in common wheat (Triticum aestivum L.).
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Primers (Supplementary Table S2) were designed according to ‘Chinese Spring’ and ‘Kenong 9204’ reference sequences [50, 57] using Premier 5.0 (Premier Biosoft, San Francisco, CA, USA).

    Amplification:

    Article Title: Molecular basis of the short- and long-term osmoregulation capability in the euryhaline unicellular eukaryote Paramecium calkinsi
    Article Snippet: .. The open-reading frames of gene TPR, as well as putative regulatory regions, were amplified by PCR and cloned into the pCE2 TA/Blunt-Zero vector using 5 min TA/Blunt-Zero Cloning Kit (Vazyme, China, Cat. No. C601-02). .. The codon-optimized GFP (723 bp, accession number AB071703 in GenBank [ ]) was inserted after the start codon ATG by ligation of ends generated by the BsaI restriction enzyme (NEB, USA, Cat. No. R3733V).

    Article Title: Functional 5Bq allele is responsible for the compact spike phenotype in common wheat ( Triticum aestivum L.)
    Article Snippet: .. Amplified target fragments were inserted into the pCE2 TA/Blunt-Zero vector using the 5 min TA/Blunt-Zero Cloning Kit (Vazyme). .. Positive colonies were sequenced by Sangon Biotech (Chengdu, China).

    Agarose Gel Electrophoresis:

    Article Title: SRBSDV-derived small interfering RNAs modulate insect vector reproduction and viral replication in Sogatella furcifera.
    Article Snippet: The primers used in this study were listed in Supplementary Table 1. dsRNA synthesis and injection Template fragments (approximately 500 bp) for dsRNA targeting SfTPS1, SfTPS2, SfTre1, SfTre2, SfTret1, SfTret1-2, SfGP, SfHK, SfToll8 and EGFP were synthesized using 2 × Phanta Max Master Mix (Vazyme Biotech, Nanjing, China), with primers listed in Supplementary Table 1. .. Following separation by agarose gel electrophoresis, PCR products of the expected size were excised, purified, and ligated into the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Nanjing, China). .. Single monoclonal plaques were selected and sent for sequencing (Tsingke Biotechnology, Beijing, China).

    Purification:

    Article Title: SRBSDV-derived small interfering RNAs modulate insect vector reproduction and viral replication in Sogatella furcifera.
    Article Snippet: The primers used in this study were listed in Supplementary Table 1. dsRNA synthesis and injection Template fragments (approximately 500 bp) for dsRNA targeting SfTPS1, SfTPS2, SfTre1, SfTre2, SfTret1, SfTret1-2, SfGP, SfHK, SfToll8 and EGFP were synthesized using 2 × Phanta Max Master Mix (Vazyme Biotech, Nanjing, China), with primers listed in Supplementary Table 1. .. Following separation by agarose gel electrophoresis, PCR products of the expected size were excised, purified, and ligated into the 5 min TA/Blunt-Zero Cloning Kit (Vazyme, Nanjing, China). .. Single monoclonal plaques were selected and sent for sequencing (Tsingke Biotechnology, Beijing, China).



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